mild hypothermia reduces expression of fas/fasl and mmp-3 after cerebral ischemia-reperfusion in rats
نویسندگان
چکیده
objective(s):to investigate the effects of local mild hypothermia on the expression of fas, fasl and mmp-3 after cerebral ischemia-reperfusion in rats. materials and methods:male wistar rats were divided into sham-operated group (sham), normothermia group (nt), and hypothermia group (ht). mcao/r model was established by longa’s method, and reperfusion was allowed after 2 hr occlusion. mild hypothermia (33±0.5℃) for 6 hr was initiated at the start of reperfusion. immunohistochemistry was performed to determine expression fas, fasl, and mmp-3. results: infarct volume was reduced in the hypothermia group (18.43±4.23%) compared with the normothermia group (24.76±5.76%) (p<0.05). in mild hypothermia group, numbers of fas-positive and mmp-3 positive cells were significantly less than those of normothermia group (p<0.05). neurological functional scores of mild hypothermia were significantly improved (p<0.05). conclusion: mild hypothermia decreases infarct volume after cerebral ischemia-reperfusion, reduces fas and mmp-3 expression, but increases fasl in cerebral ischemia-reperfusion rats.
منابع مشابه
Mild hypothermia reduces expression of Fas/FasL and MMP-3 after cerebral ischemia-reperfusion in rats
Objective(s):To investigate the effects of local mild hypothermia on the expression of Fas, FasL and MMP-3 after cerebral ischemia-reperfusion in rats. Materials and Methods:Male Wistar rats were divided into sham-operated group (Sham), normothermia group (NT), and hypothermia group (HT). MCAO/R model was established by Longa’s method, and reperfusion was allowed after 2 hr occlusion. Mild hypo...
متن کاملMild hypothermia reduces expression of Fas/FasL and MMP-3 after cerebral ischemia-reperfusion in rats
OBJECTIVES To investigate the effects of local mild hypothermia on the expression of Fas, FasL and MMP-3 after cerebral ischemia-reperfusion in rats. MATERIALS AND METHODS Male Wistar rats were divided into sham-operated group (Sham), normothermia group (NT), and hypothermia group (HT). MCAO/R model was established by Longa's method, and reperfusion was allowed after 2 hr occlusion. Mild hypo...
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متن کاملIranian Journal of Basic Medical Sciences
Article type: Original article Objective(s): To investigate the effects of local mild hypothermia on the expression of Fas, FasL and MMP-3 after cerebral ischemia-reperfusion in rats. Materials and Methods: Male Wistar rats were divided into sham-operated group (Sham), normothermia group (NT), and hypothermia group (HT). MCAO/R model was established by Longa’s method, and reperfusion was allowe...
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عنوان ژورنال:
iranian journal of basic medical sciencesجلد ۱۷، شماره ۶، صفحات ۴۵۴-۴۵۹
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